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The results are presented in Figure 2. The parameter value-based features (i.e. the inferred phylogenetic rate models themselves) are the most accurate predictors for the first and second tasks of separating primary samples from the metastatic ones.
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For two of the recurrences, the primary sample from the same patient was analysed.
MATERIALS AND METHODS: We evaluated the expression of bcl-2, bax, and p21WAF1/CIP1 in primary samples from patients with AML in the presence and absence of HGF.
Primary samples from kidney and colon were obtained from M. Haase (Dresden University of Technology).
To validate, we performed real-time PCR on 34 primary samples from a separate cohort.
The primary sample for the analysis is drawn from the 2000 2004 MEPS.
Statistics Canada releases design information for variance estimation only in the form of bootstrap weights: cross-sectional weights and longitudinal weights (adjusted for non-response) that have been created from taking numerous bootstrap samples of primary sampling units from the original sample.
External validation in 279 primary HNSCC samples from the TCGA project verified our initial findings.
DAOY, UW228-3 and MED3 MB cell lines all show high MGMT expression while MED1 cells show no MGMT expression by Western blotting, consistent with the observed expression in primary tissue samples from the corresponding tumours.
Interestingly, the genes were frequently methylated also in hematological cancer types and were therefore subjected to analyses in primary tumor samples from the major types of non-Hodgkin lymphomas (NHL) and in healthy controls.
Because the metastatic and primary samples weren't from the same individuals, and because the samples were drawn from a hodgepodge of cancers (lung, breast, prostate, colorectal, uterus, and ovary), Golub didn't really expect to see anything interesting.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com