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Several studies have shown the effectiveness of this technique under the assumption of perfect mixing of primary samples.
Quantitative real-time RT-PCR analyses show that SLC22A16 is expressed in primary samples taken from patients with acute leukemia.
The application of this system was illustrated on primary samples from an individual infected with HIV-1 followed from pre-peak viremia through six months post-acquisition.
Tissue microarray analysis showed strong staining of ROR1 in 30% or greater of primary samples in colon, lung, and pancreatic cancers.
For each fish species, equal weight of the primary samples was mixed, ground, and remixed to obtain a single homogeneous composite sample before analysis.
To validate, we performed real-time PCR on 34 primary samples from a separate cohort.
The presence of MLL rearrangements in HSPC induced the methylation profile observed in the MLL-positive primary samples.
The proposed classifier was able to correctly predict 47 of the 51 primary samples and 58 of the 61 secondary samples.
All assays for the detection of seasonal influenza viruses were validated on approximately 80 100 primary samples containing the corresponding types and subtypes.
Cloning-sequencing of the TaqI-digestion resistant bands from T-ALL primary samples uncovered the R46X mutation in 27 of 43 (62%) clones (Table 1).
Since 2007, the assays for seasonal influenza viruses have been run on approximately 1000 (N1, N2), 4000 (H3, H3) or 6000 (BM) primary samples.
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