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Primary reads.
Primary reads Image interpretation is performed with the purpose of making a radiological diagnosis, in both emergency and non-emergency situations.
Primary reads were initially trimmed off adapter sequences using Cutadapt v.1.2.1 [ 14].
Primary reads and mapped gene counts can be found at GSE59442.
Reads in a stack are primary reads, and reads that are set aside are secondary reads.
Subsequent to the pyrosequencing process, the Roche Assembler software was used for assembling the primary reads into contigs.
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Read counts per gene were determined from primary read alignments with a mapping quality of ≥ 20 (MAPQ20).
I've gotten a lot of crap over telling people that I was a former pro photographer that had long since moved to an iPhone as my primary (read: most used) camera.
In principle, there are two general modes of evaluation of images including different workflows: one is reading images with medico-legal responsibility, e.g. primary read or secondary read for an addendum or supplementary report; the other one is reviewing for comparison while follow-up studies or reviewing by other physicians.
Read counts per gene were determined from primary read alignments with a mapping quality of 20 or more (MAPQ20).
The primary reading was processed by subtracting OD readings of control wells (coated with gluthathione-S-transferase (GST) and Block Ace for recombinant GPI GST and rabbit GPI, respectively).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com