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Northern blot analysis indicates very strong signals in the corpus luteum of the cycle and pregnancy, with similar strong signals also deriving from primary preparations of follicular theca cells.
Standards for both KLK13 and actin RNAs were defined to contain an arbitrary starting concentration, since no primary preparations exist.
In conclusion, we have established a robust high-throughput screening platform that could be extended, e.g., to image-based screens or to map genotype-to-phenotype relationships [ 50] in primary preparations of MSCs.
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But they are playing tonight without the star forward Jon Brockman, who sprained his ankle last week and whose primary preparation for this tournament has been learning to walk with crutches.
Although Ha-cup1, Ha-cup4 and Ha-trypsin2 expressed in the midgut or fat body in addition to the epidermis and HaEpi cell line, the midgut and fat body cannot contaminate the cell line because they were excluded by dissection upon primary preparation of the epidermal tissue.
MT: participated in sample collection and primary preparation.
RJB was responsible for the design of the study, laboratory and statistical analysis, primary preparation of the manuscript.
KA conceived the research plan, conducted primary analysis of the USRDS datafiles, and was responsible for primary preparation of the manuscript.
For all ISG hit validation experiments, we generated lentiviral stocks that were independent from the primary screening preparations.
Replicate experiments were performed with separate primary cell preparations from at least three mice for each experiment.
Furthermore, the anti-inflammatory action of olodaterol was also assessed on human peripheral blood cells and pulmonary primary cellular preparations.
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