Sentence examples for primary polymerase from inspiring English sources
The analyzer employs a primary polymerase chain reaction (PCR) coupled to an allele-specific ligase detection reaction (LDR).
Primary polymerase chain reactions (PCR) were performed in a final volume of 20 µl containing 9.2 µl of water, 0.8 µl (10 µM) of each specific primers, 2 µl 10×PCR buffer containing 15mM MgCl2, 5 µl DNA, 2 µl 2.5mM dNTPs, 0.2 µl 5 U/µl Taq polymerase.
Moreover, we improved the cost-efficiency of the overall assay by decreasing the reagents' concentrations of the secondary (light-producing) reaction, while ensuring that they do not become rate-limiting for the processing of the PPi product of the primary (polymerase) reaction.
RpoTm is probably the primary polymerase and RpoTmp transcribes mitochondrial genes early in development [ 14].
In contrast to long patch BER, with the repair tract of two or more nucleotides, short patch repair results in the replacement of a single damaged nucleotide, and DNA pol β is the primary polymerase involved in this process.
Of the five DNA polymerases in E. coli, the primary replicative polymerase is pol III, and its catalytic subunit is encoded by dnaE.
The correlation between our biophysical data and the cell biology data reported previously suggests that the dyskerin·hTR interaction is important for the maintenance of appropriate hTR levels in the cell and is consistent with the view that dyskerin binds to hTR to direct proper processing of the primary RNA polymerase II transcript and/or to direct subnuclear localization (2, 4).
The sequences were also screened for the presence of multiple potential L1 integration sites [Yang et al., 1999], and using RepeatMasker open 3.0 (http://www.repeatmasker.org), to locate nonrepetitive DNA suitable for primary and secondary polymerase chain reaction (PCR) primer design, as shown in Figure 1A.
The C. glutamicum genome encodes seven sigma subunits (factors) of RNA polymerase: the primary sigma factor SigA (σA), the primary-like σB and five alternative sigma factors (σC, σD, σE, σH and σM).
DNA damage is the primary activator of PAR polymerase 1 (PARP1) that catalyzes the reaction of poly(ADP-ribosylation) (for review see ref. 15).
The primary structure of the polymerase includes a nuclear localization signal, a BRCA1 carboxy terminus domain, a proline-rich region, a pol β-like region and a pol X region [27, 135, 136].
