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In the Old Testament, the primary passages on the end times are in Isaiah through Malachi (including Daniel where there is much more that you can read).
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However, in the case of the Suffolk sheep, the incubation period for the TgBoPrP mice was 693.8 d in the primary passage and 457.7 d in the second passage.
All of the 89 samples that were classed as non-culturable did not show any growth in tissue on the primary passage.
Features of the disease and PrPres molecular features after a primary passage in C57Bl/6 mice have previously been described for two of the H-BSE and for the classical BSE isolate [26], [27].
For subpassages, 10% brain homogenates from Tg110 mice collected from primary passage were used as inocula.
To detach the primary passage cells, chondrocytes were digested using 0.05% trypsin:EDTA (GIBCO), and then counted using a hematocytometer.
Once the primary (passage zero) cells reached confluence (20 days), the FBS concentration was reduced to 5%.
With the exception of Figure 2D, all data for DNA-PKcs -/- cells were from primary (passage 1) cells.
Total RNA was extracted from primary (passage 4) and DF-1 (passage 285) CEF cells using TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA) following the manufacturer's instructions.
They appeared as spindle shaped cells after 3 to 4 days' culture of the primary passage, after which they proliferated rapidly.
These 3 neuroanatomic sites were severely affected in C57BL/6 mice brain after primary passage of the BSE agent as we and others have reported (1 ).
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CEO of Professional Science Editing for Scientists @ prosciediting.com