Sentence examples for primary passage from inspiring English sources
All of the 89 samples that were classed as non-culturable did not show any growth in tissue on the primary passage.
However, in the case of the Suffolk sheep, the incubation period for the TgBoPrP mice was 693.8 d in the primary passage and 457.7 d in the second passage.
Features of the disease and PrPres molecular features after a primary passage in C57Bl/6 mice have previously been described for two of the H-BSE and for the classical BSE isolate [26], [27].
For subpassages, 10% brain homogenates from Tg110 mice collected from primary passage were used as inocula.
Once the primary (passage zero) cells reached confluence (20 days), the FBS concentration was reduced to 5%.
To detach the primary passage cells, chondrocytes were digested using 0.05% trypsin:EDTA (GIBCO), and then counted using a hematocytometer.
MEFs were isolated from embryos of the indicated genotypes at E15.5 and were used as primary (passaged no more than 10 times and grown in 3% oxygen), immortalized with pLenti-lox-GFP shRNA p19-2, or spontaneously immortalized.
In the Old Testament, the primary passages on the end times are in Isaiah through Malachi (including Daniel where there is much more that you can read).
Primary (P0), passage 1 (passagessage 2 (P2) and passage 3 (P3) HACs were monolayer expanded in medium corresponding to their isolation tonicity (280 mOsm, 380 mOsm, 480 mOsm or 580 mOsm), with an initial seeding density of 6,000 cells/cm.
Finally, the primary-passage cells were harvested and replanted into appropriate culture plates.
Might a small number of 'activated' or 'aggressive' FLSs present in a primary culture (passage 1) overgrow other elements and appear as a major population in later (passage 4) cultures?
