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Gingival fibroblasts were obtained by trypsinization of the primary outgrowth of cells.
Both cytokines also inhibited cell migration in the scratch assay and primary outgrowth of CPC from cartilage tissue.
The same cytokines and concentrations were used to investigate the influence of pro-inflammatory stimulation on the primary outgrowth activity of CPC from cartilage slices.
We therefore tested two other cell types, namely a human microvascular endothelial cell line, (HMEC-1) and primary outgrowth endothelial progenitor cells (OECs) with the same panel of primers used to confirm sequencing data in HEK293T cells.
Finally, the primary outgrowth of CPC from their cartilage origin was markedly impaired by both cytokines, indicating that these pro-inflammatory factors also inhibit the process of release from the native environmental niche which is not defined so far.
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We pooled the primary outgrowths of 6 18 blastocysts, transferred the pools onto Li1, and each pool easily delivered the desired cell line.
Secondary transplantations reveal that four of the six primary outgrowths contained >5 MRUs, indicating that some of the luminal MRUs are potent and have extensive self-renewal capacity and can generate normal glands (Additional file 6B,C).
The above-mentioned experiments were conducted without neural growth factor (NGF), which terminates mitosis and induces primary neural outgrowth in PC12 cells.
Steps of malignant progression including primary tumor outgrowth, therapeutic resistance, and distant metastasis are not determined solely by genetic alterations in ovarian cancer cells, but considerably shaped by the fitness advantage conferred by benign components in the ovarian stroma.
Injected embryos were cultured for 72 hr to allow hatching and primary ICM outgrowth.
Primary tumor outgrowth was monitored every 4 days by taking measurements of the tumor length (L) and width (W).
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