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The primary omission has been with respect to synchronization.
Controls for the mGluR5 antibody experiments included both preabsorption with the control peptide (Chemicon,Temecula, CA), as well as primary omission studies, which both revealed a lack of non-specific staining (data not shown).
The primary omission is bovine spongiform encephalopathy.
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Negative controls were performed by primary antibody omission.
Primary antibody omission controls were used to further confirm the specificity of the immunohistochemical labeling.
As a negative control, adjacent sections were processed with primary antibody omission (secondary antibody only).
Specificity was confirmed by the absence of immunostaining by isotype-specific mouse antisera or primary antibody omission.
All secondary antibodies were assessed for specificity in primary antibody omission controls, fluorescence was only observed when used in conjunction with the appropriate species of primary antibody.
Primary antibody omission controls and cPLA2 knockout (KO) spinal cord sections were used to further confirm the specificity of the immunofluorescence double labeling, and secondary antibody omission controls were used to determine the degree of autofluorescence.
Primary antibody omission controls were run alongside the other samples to check for non-specific binding due to the secondary antibodies, and advanced AD human prefrontal cortical sections were run as positive controls.
Primary-antibody-omission demonstrated the specificity of the immunostaining.
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CEO of Professional Science Editing for Scientists @ prosciediting.com