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3WJdB strongly colocalized with Rab5 and Tf containing vesicles, indicating that the primary localization of this large RNA payload after 1 h incubation is early/sorting endosomes (Figs. 4, 5, and Supplementary Figs. 14 and 15).
Also Rayleigh has two seminal discoveries: the famous duplex theory, that is, below 1.5 kHz, ITD is the primary localization cue and above 1.5 kHz ILD instead, the head-shadow effect, that is, the blocking and reflection of sounds by head produce a maximum of 20 dB intensity difference.
Immunoblotting confirmed NTPD8 protein in bladder and immunofluorescence suggested a primary localization to the urothelium.
Similarly, consistent with the primary localization of CLN3p within the endosomal-lysosomal system, ER biology was not dramatically altered by the JNCL mutation but instead the results of our pathways analysis implied that lysosomal biology was strongly affected in the homozygous CbCln3Δex7/8 cerebellar cells, which was consistent with biological data in this study and others (reviewed in [9]).
Pulmonary involvement rarely occurs as a primary localization.
Primary localization of the lesion is critical for the choice of treatment strategy and type of surgery.
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Cells were washed 3× with 10% FBS/PBS and incubated with Alexa-dye conjugated secondary antibodies for 1 h to detect primary localizations.
Also, the fundal and paramedian regions were major primary localizations.
The proteins had 34 primary localizations (Table 1) and altogether 56 localizations (Additional file 2).
Conversely, in a Canadian study including 2,790 patients, Sandoval et al. found that prostate cancer patients reported a higher level of overall satisfaction compared to other primary localizations, including head and neck, brain, breast, gynaecological, lung and digestive cancers [ 7].
It is likely that our study is not representative of all patients seen in oncology practice, since we did not include some common primary localizations such as uterine or upper gastrointestinal tract cancer, and metastatic patients.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com