Tropomyosins activated, inhibited, or had no effect on motility depending on the myosin, indicating that the myosin isoform is the primary determinant of the isoform-specific effect of tropomyosin on actomyosin regulation.
This full-length Naip isoform is robustly expressed in primary macrophages from healthy human donors, but it is drastically reduced in monocytic tumor cells, THP-1, and U937, rendering them insensitive to cytosolic flagellin.
Within a given signaling pathway, identifying which NOX isoform is acting as the primary ROS source is usually accomplished by determining the relative expression level of each isoform using isoform-specific antibodies or by overexpressing the isoform of interest.
Electrophoretic mobility suggests that the lower protein isoform is equivalent to canonical EZH2α, so in primary fibroblasts EZH2 protein appears to include additional domains that are not part of the EZH2 that is expressed by cell lines.
This 50 kDa isoform is presumably sACt.
CACN-1 has three isoforms, and genetic analysis suggests that the primary isoform, CACN-1A, is responsible for regulating DTC migration (Tannoury et al. 2010).
The primary structure of actin isoforms is highly conserved, and cytoplasmic β- and γ-actin differ from each other in only four amino acids at their N-terminus (Vandekerckhove and Weber 1978b).
Different definitions for primary isoform designation have been provided in human using the APPRIS pipeline [ 49].
No expression of either Sp7 isoform was found in a range of primary and cell lines (data not shown).
For a given primary/minor isoform pair, an exon was deemed to be gained in the minor isoform if it did not overlap with any exons in the primary isoform whilst an intron was deemed to be retained in the minor isoform if it had an exon that fully overlapped an intron from the primary isoform.
