We are not aware of any relevant information regarding the inhalation toxicity of lactide, the primary individual VOC emitted from PLA filaments.
Visual inspection of the results from the primary individual-domain ALE analyses suggested that there was a difference in the domains that engaged dorsal and ventral parietal areas.
The primary analysis included individuals from both the cohort and the cross-sectional samples.
Using real-time PCR and Western blot analysis, we analyzed expression of CDKN2A/B in spontaneous and UV-induced primary melanomas from individual backcross hybrid fish.
Space-time clusters were defined as the isolation of 3 or more primary isolates from individual patients treated in the same hospital within a 2 month window displaying the same SpaType.
We analysed the methylation status BNC1, CCDC8 and GALNT9 in metastatic brain tumours and corresponding primary tumours from individual patients.
Adhering to the ethical framework, the research team anonymized and delinked the primary data from individual names, or other easily discernible identities.
In the present study, we used mRNA isolated from three human prostate cancer cell lines instead of primary tumour from individual patient as a source of polyvalent prostate cancer antigens.
Primary donkey fibroblasts were isolated and established from skin biopsies of a male individual; primary fibroblasts from Burchelli's zebra were a kind gift from Professor Mariano Rocchi (University of Bari, Italy); primary fibroblasts from Grevy's zebra were purchased from Coriell Repositories.
Immune cells from spleen, blood, and primary tumours from individual mice were isolated as described previously (Kim et al, 2008; Castro et al, 2009) Briefly, red blood cells from blood or tumour cells were lysed according to standard protocols, and the remaining leukocyte population was used for analysis.
Quantitative real-time PCR was performed on cDNA samples derived from RNA isolated from primary chondrocytes of individual rib cartilages from Hoxc8- and Hoxd4-transgenic mice, respectively.
