If a site had secondary peaks at least 0.3 the height of the primary peak, it was treated as ambiguous.
Sequence traces were used to designate individual worms as multiply infected if the secondary peak at a polymorphic nucleotide site was at least half of the height of the primary peak.
Occasional heteroplasmic sites were present in the HVR dataset, as expected for Adélie penguin HVR [ 29, 69], and these were re-scored according to IUPAC ambiguity codes when the secondary peak was >40%% of the height of the primary peak in both forward and reverse sequences.
We find the temperature structure to be strongly modulated by tides and gravity waves, but average profiles typically show a primary mesopause height close to 100 km with temperatures around 180 K, and a tendency for a secondary minimum of about 185 K to occur close to 90 km.
There was a wide variation in overall dwarfing phenotype (DW%), increase in scion TCA, primary axis height, node number and the number of flowers in the spring of year 2 (Supplementary Figure S1).
There was evidence in proportional growth of girth, number of primaries and height when coffee plants are under organic manure (p < 0.05, Table 3; Fig. 3a, b, c).
There was no significant partitioning in the growth of girth, number of primaries and height when coffee plants are irrigated with high, medium and low amounts (p > 0.05, Table 4).
The two primary determinants of height growth are the number of height growth units (the node plus its subtending internode) produced during each growing season and elongation of the internodes.
The primary outcomes were height gain (cm), weight gain (kg), the absolute gain in height-for-age Z score (HAZ) and weight-for-age Z score (WAZ), stunting (<-2 HAZ) and underweight (<-2 WAZ).
