Sentence examples for primary gate from inspiring English sources

A primary gate based on physical parameters (forward and side scatter) was set to exclude dead cells or small debris.

A primary gate based on physical parameters (forward and side light scatter, FSC and SSC, respectively) was set to exclude debris and cell aggregates.

The sorting primary gate was set to properly capture nuclei based on size and density, while secondary gates were set based on fluorescence signals.

As with the established Blantyre count method and in contrast with the Panleucogate method, threshold for acquisition was set on the FL1 (CD45-FITC) channel rather than forward light scatter, the side light scatter parameter was set to a linear rather than a log scale to aid discrimination of lymphocytes and monocytes, and a total lymphocyte rather than total leucocyte primary gate was utilized.

"It's the primary gating factor," Mr. Larner said.

CD45-positive lymphocytes and monocytes are identified by primary gating on bright CD45 fluorescent cells in a CD45xside scatter dot plot scattergram.

In most K+ channels, the primary gating mechanism involves an opening of the bundle crossing to permit the flow of ions across the membrane (Loussouarn et al, 2002; Yellen, 2002; Swartz, 2004).

Importantly, we have also used them to demonstrate that the primary gating mechanism within TREK-1 exists close to, or within the selectivity filter and that unlike most other types of K+ channels, gating of these channels does not involve opening and closure of the helix bundle crossing.

Together, these results provide a convincing argument that the processes of gating and permeation are intimately coupled in TREK-1, and that the selectivity filter is not an isolated structural domain, but instead acts as the primary gating mechanism for activation in TREK-1 channels.

Primary gates for small pNPC and bigger notochordal cells (pNC) were defined based on primary cells digested from discs lacking a notochordal cell phenotype, including bovine and a whole human lumbar IVD obtained from a spinal fusion (ethical permit has been obtained from the local ethical committee).

This study reveals that in contrast to most other K+ channels the primary gating mechanism in the K2P channel TREK-1 does not involve opening and closure of the cytoplasmic bundle crossing, but takes place close to or within the selectivity filter.