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The assignment of the O. novo-ulmi Yeast LMW ESTs with known identities (n = 880) into functionally related groups yielded 20 primary functional categories (Table 2).
Primary functional categories were associated mainly with protein, carbohydrates, amino acids, DNA and RNA metabolism, cofactors, cell wall and capsule and membrane transport.
Each of the eight primary functional categories that represented more than 4.5% of all identified ESTs were categorized to the secondary level within each category (Table 3).
Primary functional categories were similar to those of other gut microbiomes and were associated mainly with protein, carbohydrates, amino acids, DNA and RNA metabolism, cofactors (vitamins, prosthetic groups, pigments), cell wall and capsule and membrane transport.
Compared to leaf tissue, up-regulated TDFs were found more abundantly in the root across the five primary functional categories- unclassified, metabolism, stress/defense, signal transduction, and gene regulation.
Of the eight primary functional categories examined at the secondary level, FunCat 11 (Transcription) exhibited the least complexity, with 72.75 standardized functional assignments of ESTs in three subcategories, primarily RNA synthesis (11.02, 86.25%), RNA processing (11.04, 11.00%) and RNA modification (11.06, 2.75%).
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Of the 29 primary level MIPS functional categories, "Metabolism" and "Energy" are two significantly over-represented terms among the 966 L/C regulated genes in wild-type (Table 2a).
TUSs were thus assigned to primary and sub-GO functional categories.
Assembled sequences were thus assigned to primary and sub-GO functional categories.
The endo/ecodormancy transition (SEP to NOV) was characterized by a further decline in transcripts participating in cellular processes as well as primary and secondary metabolism functional categories, similar to what was observed in the para/endodormancy transition.
In the following sections, we discuss the differences in the regulation of transcripts in the functional categories of primary and secondary metabolism, peptidase and hydrolases, transporters, and genetic information processing observed in Ms and Hv larvae that fed on defenseless plants.
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