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Taking into account the secondary de-excitation processes of primary fragments with the GEMINI code, these energy peaks fall to about 0.45 MeV/u.
For LC/MS/MS, secondary masses are also created from the bin/group of all secondary ions across the samples and the averages represent the secondary ions from one of the primary fragments of the chemical entity.
The modification of the nucleobase weakens the N-glycosidic bond, favouring a depurination of ON-1* under participation of the neighbouring O-atom to afford the primary fragments ON-1 A ON-1 A under mild ionisandon conditions (Fig. 8).
IRMPD is often regarded as a more efficient fragmentation technique, as it acts upon not only the isolated precursor ions but also upon their primary fragments, thus producing additional smaller fragment ions.
It has been reported that electron ionization (EI) of certain scaffolds involves complex fragmentation due to the broad spectra of internal energy of the molecular ion peak (M+∙) which suppresses the M+∙ and other primary fragments containing the structural information [ 45].
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Each derived fragment should then be assigned to the same database server on which the primary fragment with the matching join attribute values resides.
the primary fragment and its derived fragments are hence assigned to the same server and the server information in the lookup tables is inserted accordingly.
the sizes w i are now computed as the sum of the size of the primary fragment F i plus the size of any derived fragment G i. solving the BPPC results in a placement where the primary fragment fits on the server together with all its derived fragments.
We track the number of significant peaks (which correspond to primary fragment ions) while still noting but preserving the non-signficant peaks during filtering.
However, none of these primary cleavage fragments could be detected in full length (65 155 kDa) among the analyzed spots, which contained only smaller VTG fragments of 18 27 kDa, possibly as a consequence of a residual proteolytic activity during the preparation of whole-animal extracts.
Playback techniques were used to detect individuals in the continuous primary forest, fragments of 1, 10 and 100 ha, and in two matrix habitats of secondary forest dominated by Vismia spp. and Cecropia spp. Sampling points in secondary forest areas were distributed at 50, 250 and 500 m from the continuous forest.
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