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Thus, we set out to develop a primary culture model de novo.
In this primary culture model, we have been able to provide a proof-of-concept that 1) candidate lung CSC are present in this milieu, 2) candidate lung CSC can be maintained over time in this primary culture environment, and 3) that we can live sort candidate lung CSC from these primary cultures to evaluate their phenotype in various bioassays.
A rat ENS primary culture model confirmed this expression.
A laryngeal cancer-derived secretory/releasing protein database was established on the basis of a novel serum-free primary culture model.
We tested whether FK866 and NMN also influence the Vincristine primary culture model of Wallerian-like degeneration, a pathology underlining chemotherapy-induced peripheral neuropathy.
The importance of cell cell contacts has been recently pointed out in a primary culture model close to ours (Kondo et al, 2011).
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There is evidence for tissue-specific hormonal effects to be maintained more properly in tissue culture when compared to primary culture models [ 21- 25].
Caspase 3 activation has been associated not only with apoptosis but also with nonapoptotic pathways of synaptic pruning and LTP deficits as shown in recent work on rodent models and primary culture models of Alzheimer's disease biology.
In summary, our results argue for the ongoing development of the MPE-primary culture model, and set the stage for correlating observed phenotypic differences with distinctive molecular signatures.
However, the reproducibility of our results across multiple primary cell isolates at each stage indicates that this primary cell culture model has the necessary fidelity for demanding mechanistic studies.
In summary, we have developed a novel primary cell culture model of a septic alveolar epithelium that incorporates the complexity of septic injury in vivo with the specificity of an in vitro monoculture study.
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