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In contrast, although Lgr5- cells expand into primary colonies, colony-forming efficiency immediately dissipated upon passaging.
D0PPA blastocysts were able to form tertiary trophoblast colonies but D6PPA and control groups were not able to grow beyond primary colonies.
The average sizes of secondary colonies are smaller compared with primary colonies after a 7-day culture.
WT C. parapsilosis was transformed with the plasmid pSFS2Fas2 by electroporation resulting in more than 35 primary colonies.
Second, twelve single primary colonies were isolated from 67:20 and 67:21, and subcultured in parallel for 50 passages, transferring one single colony in each step.
The numbers of primary colonies from each sample were 1, 5, 2 and 1, respectively.
Similar(38)
The survey area was placed between the prehistoric urban capital of Tiwanaku and its primary colony in Moquegua, a valley located on the Pacific watershed approximately 325 km away.
Primary colony-formation assays in methylcellulose demonstrate effects on tumorigenic potential of these leukemia cells.
An intriguing issue with the colony assay is that after dissociation, few primary colony cells can replate and form secondary colonies [25].
The closest approximation to estimating the frequency of stem cells is derived from the primary colony forming efficiency (CFE) assay, which enumerates the number of Colony Forming Units-Fibroblast, approximately 1 in 5 of which are stem cells [16].
CD146 appears to be a marker for the in situ counterpart of the primary colony forming unit fibroblast, [14] and thus, possibly an indirect estimate of the stem cells in BMSCs.
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