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The primary coating failure mechanism was microchipping.
An optical fiber consists of a core region and a cladding layer surrounded by a protective primary coating.
For the ELISA, we used a primary coating antibody generated against human apoB-100 (mAb47, kindly supplied by J. Witztum, University of San Diego, USA), in a concentration of 2 mg/well IgG protein in TBS/EDTA/BHT and a secondary biotinylated polyclonal antibody raised in goat against human ApoB in a concentration of 4 ug/well in 1.5% BSA/TBS/0.1% Tween.
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Most routinely, 0.2 μg of protein was coated per well, but for quantitative comparisons of relative specificity of polyclonal reagents for each of native and mCRP, the primary coat protein was varied from 0.001 μg/well to 0.8 μg/well.
ELISA analyses were performed using various primary coat concentrations and various dilutions of defined monoclonal anti-CRP reagents (Ying et al. 1989, 1992) and a representative polyclonal anti-CRP reagent.
Brown Swiss do have some similar markings as Jerseys do, but the primary coat colouration of a BS is greyish-brown, not fawn.
The synthesis of primary PPy coating was carried out from aqueous oxalic acid solution and the synthesis of PIn top film was obtained from 0.15 M LiClO4 solution in acetonitrile.
The two telescopes have been optimized for observations at infrared wavelengths by having their primary mirrors coated with silver, which does not emit as much thermal infrared radiation as the more commonly used aluminum coatings.
In remarks after winning the primary, Mr. Coats made clear that he intended to strike very conservative themes on spending, the scope of government and national security while trying to tie Mr. Ellsworth to President Obama and the House speaker, Nancy Pelosi.
The formation of primary grain-coating chlorite is similar to the formation process of oolitic lime, which requires highly hydrodynamic conditions and for grains to constantly adsorb flocculent precipitates from the water (Yao et al. 2011).
A high-performance yarn electrode material, cotton/graphene/polyaniline, is synthesized by coating primary fiber cores inside cotton yarns with graphene sheets and followed by further growing polyaniline nanowire array layers through in situ polymerization of aniline.
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