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In RE-ChIP assays the primary ChIP (for Ctcf) was performed accordingly to the standard protocol starting from 2×10 cells for each IP.
This description serves as the primary chip design verification tool at both the functional and the layout levels.
We focus on two stages of analysis: (1) visualization of the primary ChIP data and assessment/refinement of the binding region calls, and (2) identification and characterization of regulatory motifs found within the refined binding regions.
For sequential ChIP (re-ChIP) assays, complexes from the primary ChIP were eluted twice with 10 mM dithiothreitol for 20 minutes at 37°C, diluted 10 times with re-ChIP buffer (20 mM Tris-HCl pH 8.1, 0.1% Triton X-100, 2 mM EDTA, 150 mM NaCl) followed by re-immunoprecipitation with the indicated second primary antibody, and then again subjected to the ChIP procedure.
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Device makers typically buy their primary chips from specialized microprocessor companies.
Apple's laptops and desktops run on Intel chips, while Samsung has been selling Apple the primary chips for the iPhone.
Intel and Advanced Micro remain in a pitched battle to provide the processors, the primary computing chip in PC's.
In both cell culture and primary adipocytes, ChIP results show that PRMT5 associates with PPARγ2 and PPARγ2-responsive promoter sequences, activating adipogenesis.
Without the GRAT or the family partnership, they will have lost their primary bargaining chips.
Their primary trade chips are Robbie Ray and Zack Greinke, and Ray has continued to draw interest from Houston and Atlanta as the offseason progresses and the free agent market thins.
Primary microarray, ChIP-seq and RNA-seq data have been deposited in the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus under accession number GSE51894.
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