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Chitin and chitosan supported adhesion and differentiation of primary chick dorsal root ganglion neurons in vitro.
Neural cell compatibility of chitosan and N-acetylated chitosan was tested using primary chick dorsal root ganglion (DRG) neurons.
In vitro cytotoxicity studies with both fibroblasts and primary chick neurons demonstrated that the proposed polyanhydride blend was non-cytotoxic.
The peptide modified P HEMA-co-AEMA) scaffolds were easily fabricated in aqueous conditions, highly reP HEMA-co-AEMAll-defined, and enhanced neural cell adhesion and guided neurite outgrowth of P HEMA-co-AEMAdorscaffoldsganglia neurons relative to non-peptide-modified controls.
The procedure is carried out in primary chick embryo fibroblasts grown in serum-free media and was applied to the production of three rMVA/rFPV pairs expressing the H5N1 avian influenza antigens M1, M2 and NP.
An SDF1-induced rise in cAMP has been observed in cultured primary chick retinal neurons [8].
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Cellular interactions of primary embryonic chick dorsal root ganglia (DRGs) were compared on the hydrogels.
Cell death induced by trophic factor withdrawl in primary cultured chick dorsal root ganglia (DRG) neurones is inhibited when crmA (cytokine response modifier A gene from cowpox virus that encodes an inhibitor of caspase-1) or bcl-2 are overexpressed, and in chick motoneurones treated with the caspase-1 inhibitor YVAD.
Immunohistochemistry on cryostat sections was performed by sequential exposure to primary antibodies: chick anti-GFP (Abcam, Cambridge, MA), guinea pig anti-SLC17A7 (gift from the Jessell lab), rabbit anti-SHANK1 (Synaptic Systems, GmbH, Goettingen, Germany), and fluorophore-conjugated secondary antibodies (Jackson Immunoresearch, Laboratories, West Grove, PA and Invitrogen, Carlsbad, CA).
Using the 4B6 antibody and the G9 riboprobe derived from our subtraction, we report the detailed distribution of CRTL1 protein and crtl1 transcripts during primary neurulation in chick embryos.
To this end, we explored the effect of D-Asp (D-aspartate) on Ser-2448 mammalianmalian target of rapamycin) phosphorylation in primary cultures of chick Müller glia.
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