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The objective of this preclinical study was to provide a primary characterisation of the in vitro and in vivo activity of masitinib (mesylate salt) and to compare it against the benchmark protein TK inhibitor imatinib.
The primary characterisation of all the lines in this paper was carried out without knowing the identity of the genes tagged, as gene identity was released by the Consortium post-annotation in Flyprot.
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This study addresses pain as a primary focus, as better characterisation of pain may pave the way for targeted intervention.
Characterisation of primary cultures PAP60 and MIH55 determined their highly proliferating advantage, transforming capability and tumorigenicity in vitro and in vivo.
In summary, the benefits from M&S methodologies at the non-clinical stage include the prediction and characterisation of primary PK parameters (PBPK models) and pharmacodynamic properties (PBPK-PD models).
Specific attention should perhaps be given to cases of melanoma, endometrial cancer, and colorectal cancer in the individual or in the family, since these tumour types may be prevented through screening programmes, whereas the occurrence of multiple primary STS needs further characterisation in order to clarify its causes.
However, none of the above studies combined extensive characterisation of the primary cells.
Detailed description of the isolation, culturing and characterisation of these primary cultures have been published elsewhere (Sanchez et al, 2005; Castellon et al, 2006, 2005).
Use of primary care electronic records allowed characterisation of the clinical features, and prognosis over time, before and after a diagnosis of pancreatitis.
First, the molecular characterisation of the primary tumour combined with (neo -adjuvant treatmeneo -adjuvantd recurrence informatreatment thistoryise for hypothesis-generandng findings in terecurrencedinformationprognostic biomarkers in early-stage disease.
In order to optimise treatment strategies, the phenotypic properties of DTCs – the surrogate marker of minimal residual disease – should be taken into account in addition to characterisation of the primary tumour.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com