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Although DNA-PK is implicated in a variety of functions from activation of innate immunity [ 23] to regulation of gene expression [ 24], its primary cellular function is to initiate NHEJ.
For each gene identified, the primary cellular function (Function), the common name (Name), the Genbank Accession number (GenBank), the chromosomal locus (Locus), the p-value indicating its significance that its expression is affected by NF-κB [Pr(F ], and its Cluster location (Cluster).
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Primary cellular functions of these genes are listed in Table 4.
The exact role of FXN and its primary cellular functions in sensory neurons and cardiomyocytes – the cell types that are mostly affected in FRDA – remain largely unclear.
Besides its well-characterized role as a key signaling component during pathogen attack, a broader function for NDR1 is strongly suggested by the data in mediating primary cellular functions in Arabidospsis through maintenance of PM-cell wall connections [ 44].
Therefore, many of the genes identified by CoNECT that are expressed in smaller cellular populations of a tissue may in fact be important primary regulators of cellular function.
We first investigated the ability of the deletion mutants to function as primary cellular receptors for human adenovirus serotype 5 (Ad5).
The Human Protein Reference Database (HPRD) [ 170] was used to determine molecular function and primary cellular localization.
As identified by IPA, Cell-to-Cell Signaling and Interaction, and Cellular Movement were the primary molecular and cellular functions that are likely to be affected in shPTK7 cells.
Interestingly, a recent genome-wide study comparing orthologous groups of genes in four model genomes (Arabidopsis, poplar, rice, and P. patens) found that genes with stress-responsive expression patterns (including defense) are more likely to have undergone lineage-specific tandem duplication than genes involved in primary metabolic and cellular functions [ 58].
Such regulatory mechanisms may link N-CAM binding function with other primary cellular processes during the embryonic development of pattern.
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