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Primary cells and primary batteries.
But primary cells taken directly from an organism do not.
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Additionally, candidate regions were annotated with 15 state chromatin segmentation in breast vHMECs (E028), mammary epithelial primary cells (HMEC, E119) and breast myoepithelial primary cells (E027).
More informative is the effect of deletion of CIZ1 in primary cells.
These were routinely grown to 90% confluence then passaged as for primary cells.
This enhancement is robust and occurs in both immortalized and primary cells.
Thus, in our study, nucleofection was used to transfect the BAC vector into bovine primary cells.
Pathania, S. et al. BRCA1 haploinsufficiency for replication stress suppression in primary cells.
Recently, it was reported that some primary cells could be efficiently transfected by nucleofection19.
Stewart, S. A. et al. Lentivirus-delivered stable gene silencing by RNAi in primary cells.
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