The primary cell types of tendons are the spindle-shaped tenocytes (fibrocytes) and tenoblasts (fibroblasts).
Two primary cell types make up the pineal gland.
We demonstrated its utility in several cultured and primary cell types.
Whereas viral strategies are time consuming and involve safety risks, non-viral methods proved to be inefficient for most primary cell types.
Macrophages in particular are believed to be among the "first responders" and primary cell types that uptake and process nanoparticles, mediating host biological responses by subsequent interactions with inflammatory signaling pathways and immune cells.
Using commercial magnetic beads, we achieved efficient purification of HIV-1-infected cells (i.e. purity > 85% and recovery > 90%) from diverse primary cell types at early time points following infection.
These effects involve the release of specific CKs and GFs within the local environment [7], resulting in recruitment of two primary cell types to the site, inflammatory cells and progenitor cells (MSCs and HSCs) [19], which in turn regulate the subsequent healing processes.
The goal of this study was to determine the elastic and viscoelastic properties of three primary cell types of mesenchymal lineage (chondrocytes, osteoblasts, and adipocytes) and to test the hypothesis that primary differentiated cells exhibit distinct mechanical properties compared to adult stem cells (adipose-derived or bone marrow-derived mesenchymal stem cells).
In this report, we show efficient transfer of DNA expression vectors and siRNA oligonucleotides into a variety of primary cell types from different species utilizing the Nucleofector technology, including human B-CLL cells, human CD34+ cells, human lymphocytes, rat cardiomyocytes, human, porcine, and bovine chondrocytes, and rat neurons.
The general design of this family consists of carbohydrate residues copolymerized with oligoethyleneamine units, which have proven to be an effective motif that promotes polyplex formation, efficient cellular internalization, high gene expression and low cytotoxicity with cultured cell lines and primary cell types.
Two primary cell types (human mesenchymal stem cells (hMSCs) and human dental pulp cells (hDPCs)) were used to elicit the changes in total DNA content, integrin subunit levels, phosphor-focal adhesion kinase (pFAK) levels, and mitogen-activated protein kinase (MAPK) signaling pathway activity at the cell attachment stage.
