Sentence examples similar to primary cell specific from inspiring English sources

Primary cell-specific monoclonal antibodies (mAbs) were used in combination with goat polyclonal antibody specific for ADORA2B (1:50; Abcam, Cambridge, UK) as previously described [ 15].

Primary cell-specific mAbs were applied for 1.5 hours, and the sections washed again before bound primary antibody was detected with AlexaFluor488-conjugated goanti-mouseuse IgG (Invitrogen) for 1.5 hours diluted in RPMI/10% FCS containing 1.25 mg/ml Hoechst 33342 nuclear stain (Molecular Probes® Invitrogen, Eugene, Oregon, USA).

Sections were incubated with the primary, cell-type specific antibody for 60 minutes (CD3, polyclonal, or CD103, monoclonal, clone Ber-ACT8, both Dako) at room temperature followed by biotinylated goat anti-rabbit or horse anti-mouse (Vector Laboratories) secondary antibodies, respectively.

The primary cells exhibiting specific HIF-1α, iNOS, and MMP3 immunoreactivities were morphologically consistent with macrophages, mainly in inflammatory infiltrate and invasive pannus of the inflamed synovial joint.

Secondary cell walls are deposited on top of the primary cell wall in specific tissues (e.g., vessels and fibers) to provide rigidity and strength.

Although obtained in a single primary cell line this specific finding may corroborate our hypothesis that chromosomal alterations involving Chr17 in breast cancer may be indeed very complex and merit further investigation in primary cell cultures obtained from carcinomas of different phenotypes.

Taken together, these results suggest that following co-transfection of pBS-DS-hIgG with pBS-Ova2.8-Cre in oviduct primary cultured cells, specific expression of Cre driven by the Ova2.8 promoter excises the stuffer genes in pBS-DS-hIgG and induces strong expression of hIgG in oviduct cells.

To assess the absence of differentiation towards germinal layers, cytokeratin, early mesoderm, embryonic myosin and neural precursor cell specific primary mAbs (DSHB) were used [ 65] (Table  4).> For each graft, 2 to 3 male ES-like colonies were harvested from the Petri dish, disaggregated using trypsin and pooled together, as previously described [ 16].

A successful combination of sequence capture and deep sequencing of cDNA generated from total or poly(A -purified RNA -purifiedicient coveRNAe has the potential to simultaneously test for ASE, in specific primary cell subsets, most findings identified by Gwithcansufficientd that the expression levels of the transcoveragere sufficient and haserozygous SNPs are available.

Western immunoblot analysis was performed on homogenates of myometrial tissues and primary cell cultures with monoclonal antibodies specific for protein kinase C isozymes.

However, using immunocytochemistry only a P2Y2-receptor-specific fluorescence was detected in the membrane of either Kyse-140 and primary culture cells, whereas neither Kyse-140 cells nor primary cell culture preparations showed any specific staining for P2X4 receptors.