Sentence examples for primary cell populations from inspiring English sources

Currently, we are routinely using primary cell populations to validate new questions and reagents that subsequently will be addressed in organ culture systems.

Heterogeneity is a hallmark of cell biology, and is strongly evident in primary cell populations isolated from the same tissue [2].

As culture conditions become better defined, either to support the specific expansion of homogeneous primary cell populations or to support the survival and expansion of complex primary cell mixtures derived from disrupted mucosal surfaces, so the utility of primary cell populations should increase.

The experiments with primary cell populations are at least one step further removed from reality than tissue pieces in organ culture but primary cells do provide biological value relative to standard immortalized epithelial cell lines.

This method allows for highly efficient transfection of DNA or siRNA into isolated primary cell populations or within mixed cell suspensions as well as into other cell populations that are considered difficult to transfect.

In several cases, mixed fibroblast/epithelial primary cell populations expanded concurrently in adjacent but non-overlapping zones on the same transwell membrane and preferential attachment of C. albicans colonies to fibroblasts was consistently observed.

Since COMT proteins have been detected exclusively in the neuropil of the cerebral cortex (Kaplan et al, 1979; Karhunen et al, 1994; Lundstrom et al, 1995) and neurons are the primary cell population producing COMT mRNA, it is reasonable to assume that COMT is located at dendrites and/or spines in the prefrontal cortex (Matsumoto et al, 2003).

While it has been demonstrated previously that more differentiated cells demonstrate a lower reprogramming efficiency [11] and different somatic cell types possess differential reprogramming ability [12], [13], no study to date, to our knowledge, has identified subpopulations of cells within a primary cell population possessing differential reprogramming potential.

An alternative to generating immortalized lines of myogenic cells is the utilization of primary cell population enriched for myogenic precursors.

We recommend using a primary cell population that is abundant and easy to isolate using FACS, e.g., CD4+ T cells from mouse spleen or a passaged cell line.

This did not occur in Adeno-Cre-infected cells from WT mice or in the fibroblast-like primary cell population from dio3fl/fl mice.