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Previous studies have shown that activation of the primary barrel cortex is crucial for texture coarseness discrimination (Guic-Robles et al., 1989; Carvell and Simons, 1990; Guic-Robles et al., 1992), yet despite its importance the functional organization and texture coarseness coding in the S1 barrel cortex are largely unknown.
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Thalamocortical slices of 300 µm thickness containing the primary somatosensory (barrel) cortex were sectioned with a vibratome (VT1200S; Leica).
We first analysed how chemical fixation altered the volume of the neocortex by comparing fresh and chemically fixed brain sections cut through mouse primary somatosensory barrel cortex.
Nevertheless, tangential organization, and thus somatotopy within the primary somatosensory barrel cortex, seems to be maintained (Caviness 1976; Wagener et al. 2010; Guy et al. 2015).
For the classification of the different L6B neuronal subtypes, 155 neurons were randomly patched in acute slices of the adolescent (P17 24) primary somatosensory barrel cortex of the rat.
The primary somatosensory (barrel) cortex is effectively driven by weak but near-synchronous thalamocortical (TC) input to the granular layer 4 (Bruno and Sakmann 2006).
Thalamocortical slices (300 µm; Porter et al. 2001) containing the primary somatosensory (barrel) cortex were cut using a vibratome (Leica VT1200S).
The lemniscal pathway relays whisking and touch information from the ventral posteromedial thalamic nucleus to layer IV of the primary somatosensory "barrel" cortex.
Cells were counted in the primary somatosensory (barrel field) region of the cortex (Paxinos and Franklin 2001) in a defined area (1000 µm width) spanning the pial white matter extent of the cortex.
An example for this type of experiment is shown for the labeling of reciprocal projections between the VPm division of mouse thalamus and the primary somatosensory barrel cortex (S1-BC; Fig. 1d).
The aim of the present work was to make quantitative predictions about the innervation of excitatory cortical neurons by TC boutons in a column of primary somatosensory barrel cortex.
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