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These findings support the idea that the gastrulation arrest phenotype is a true result of XMeis3 loss-of-function and that XMeis3 is required for patterning (a part of) the primary axis in Xenopus embryos.
To presume that distinct Hox expression domains have independently evolved to differentiate axial regions along the primary axis in both the Cnidaria and the Bilateria is clearly less parsimonious.
Our analysis is consistent with that of other authors that have argued the primary axis in the bird wing exceptionally develops into digit 3, rather than digit 4 [28] [29], [29].
From the second population, three QTLs controlling the height of the primary axis in year 1 were identified.
Four QTLs controlling the number of nodes initiated on the primary axis in year 1 were identified.
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Wnt4 is one example of a Wnt paralog expressed at the right stage and location in developing Nematostella[ 44] and amphioxus [ 45] to be involved in patterning along the primary axis; yet, in the absence of specific knockdown or knockout data, no case for the specific involvement of Wnt4 can be made.
It is therefore reasonable to hypothesize that there might be some separation of the two groups along the primary axis; however, in spite of the slight difference in WPLCP detected using the phylogenetic framework described above, there were no differences detected in traits associated with the LES (Fig. 1).
In particular, we suggest that loci associated with the primary axis of variation in this cross may have played a role in distinguishing morphological species boundaries for LF and TRC, as well as distinguishing species across the radiation as a whole.
By 1993, Alcoff and Potter noted that feminist epistemology should no longer be taken "as involving a commitment to gender as the primary axis of oppression, in any sense of 'primary,' or positing that gender is a theoretical variable separable from other axes of oppression and susceptible to a unique analysis" (Alcoff and Potter 1993, 3 4).
Our findings suggest that the primary axis of trait variation in light-limited, lowland tropical forests was identical to the LES and corresponds with the shade tolerance continuum.
The same directionality of trait loadings and similarly high percentage of variance explained by the primary axis were also found in species at a given elevation [see Supporting Information—Table S3 ].
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