Exact(53)
We identified two hits with validated structures and confirmed activity in the primary assay (IC50 values = 322 nM and 7.7 μM) and two orthogonal MEKK2 biochemical assays.
The primary assay analyzed RET exons 10, 11, 13, 14, and 16 with a total of seven reactions using eight unlabeled probes.
The primary assay genotyped wild-type exons, a common exon 13 polymorphism, and an exon 16 mutation, whereas other RET sequence variation was detected.
In order to identify small molecules with MEKK2 inhibitory activity, we screened a collection of known kinase inhibitors using a high throughput MEKK2 intrinsic ATPase enzyme assay and confirmed activity of the most potent hits with this primary assay.
Again the length of incubation time may be selected simply to parallel that of the primary assay.
These cytotoxicity assays are usually run to triage compounds which appear active in a cell-based primary assay against a target of interest.
Similar(7)
The flow cytometric intracellular cytokine staining assay is one of the primary assays for enumerating vaccine-induced T cells in vaccine clinical trials.
All the target compounds were evaluated in vitro for their antifungal activity against six phytopathogenic fungi, some compounds exhibited potential activity in the primary assays.
Some of the analogues displayed good activity in the primary assays, and the seven compounds 10b, 10c, 11e, 13e, 21, 22c and 22e (shown in Figure 1) were identified as the most promising candidates for further study.
Fertility and male male interactions were assayed as in Primary Assays above.
Tau aggregation has been defined using multiple techniques, but three primary assays are traditionally used.
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