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Rhodamine B was the primary analyte for the transfer across the SLM.
Screening for MCAD deficiency was accomplished using C8 acylcarnitine (octanoylcarnitine) as a primary analyte with C6 (hexanoylcarnitine), C10 (decanoylcarnitine) and C10 1 (decenoylcarnitine) acylcarnitines as secondary analytes.
As amodiaquine undergoes a rapid and extensive conversion to its active metabolite, desethylamodiaquine was considered the primary analyte [ 9, 10, 18].
21 For example, in the United States, the primary analyte used for congenital hypothyroidism (CH) newborn screening has been thyroxin (T4), because most newborns are screened before the optimal time for screening with thyrotropin (thyroid stimulating hormone, TSH).
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However, the observed FP rate in the Minnesota population has matched the average performance of all primary analytes that have caused FP outcomes over a 6-year period (0.0025%; range: 0.0002 0.0218%; n = 24) for the conditions detected by MS/MS.
We measured and reported the disposition of the parent compounds and their metabolites separately, but we considered the primary analytes (total DHA for AS, and DEAQ for AQ), as the biologically and clinically relevant indicators of drug absorption.
The amine derivatization reagent p-nitrophenol-2,5-dihydroxyphenylacetate bis-tetrahydropyranyl ether (NDTE) was used in conjunction with capillary electrophoresis (CE) and electrochemical detection (EC) for the pre-separation derivatization of primary amine analytes present in aqueous solution.
From April 2006 through to September 2007, samples that had an elevation of any of the above analytes or ratios were flagged in the information system as a potential positive result, regardless of the concentration of the primary C8 acylcarnitine analyte.
Between the two methods, Method I is more sensitive, and Method II is more suitable for producing a general assay by changing a primary antibody for another analyte.
The combination of MEPS and UPLC provides a method for the primary screening of the analytes in 18 min with excellent recoveries at three concentration levels, ranging between 80 and 104% (relative standard deviation <11%).
Analytes of primary metabolism revealed the largest diversity regarding compartmental class assignments as these compounds are crucial constituents for many pathways.
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