All primary analyses will, to as great an extent as possible, be conducted on an intention-to-treat basis.
The biospecimen repository at the Harvard School of Public Health in Boston, Massachusetts, USA, is responsible for performing primary analyses on all specimens, as well as storing remaining specimens from patients who have given separate consent in a biorepository.
While post hoc analyses are not as powerful as primary analyses, ethical considerations led us to pursue this course instead of setting up a new interventional study in children, since relevant data had already been collected.
As noted earlier, all assessments include a set of common core data elements, which are the primary focus the present analyses, as well as specialized items unique to that service setting.
Although we cannot formally exclude that the two lesions represent the occurrence of two independent primary ATRTs, our genetic analyses as well as the clinical history of the patient, characterized by the presence of metastatic spread since the occurrence of the primary tumor, make this hypothesis very unlikely and support a clonal origin of the two lesions.
We performed all analyses using SPSS software (version 20, IBM Corp .. We performed the aforementioned analyses as primary analyses.
We restricted the primary analyses to AD as the most common type of dementia.
Here, data in the results section are taken from the primary analyses unless specified as being from secondary analyses.
The beta coefficients (regression slopes) for C677T from the primary analyses were used as the observed effect size, and the corresponding squared standard error (SE) was treated as known variance in the diagonal of the variance covariance matrix R. HW equilibrium was tested using Fisher's exact test as implemented in PEDSTATS [Wigginton and Abecasis, 2005].
Second, for the main model with dichotomized outcomes primary analyses using country as a fixed effect, were repeated using a generalized mixed model approach with country as a random effect Second, to test whether the effect of congenital toxoplasmosis on outcomes differed across countries, we derived separate multivariate models for each country.
