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It is notable that our method correctly distinguished main domains from swapped domains for almost all of these cases, such that the primary alignment of every DSCO pair was generally based on the main domains.
We thank Gillian Gibb for the primary alignment of avian mitochondrial genomes and software-human interface assistance and Warwick Allan for help with analyses.
A primary alignment of all sequences was created using MAFFT [ 61] implemented in Geneious pro (version 5.6.4; [ 62]) and this alignment was improved manually using BioEdit (version 7.0.5.3 [ 63]).
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Primary alignments of codons were performed with PRANK (Loytynoja and Goldman 2005) and then visually inspected.
Among other advantages, molecular morphometrics makes it possible to identify characters informative about parsimony that are not found in the primary sequence [ 16, 17], and to correct primary alignments on the basis of secondary structure information [ 3].
The primary alignment was done using data on the secondary and tertiary structure of cyclins [ 27].
(D) Primary sequence alignment of members of the filoviridae family.
The primary sequence alignment of hTLR10 and its template was used to construct the final model (Figure 1).
Furthermore, a primary structure alignment of FtHPPK-DHPS from various F. tularensis strains reveals a wild type sequence, and no detrimental mutations have been acquired in the attenuated LVS strain we are using.
Protein primary sequence alignment of UCPs and ADP/ATP carrier (AAC) were performed using Clustal Omega [ 26].
Primary sequence alignment of AANATA with other D. melanogaster AANATL enzymes identified a number of conserved residues that might function in catalysis.
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