Deregulation of allosteric inhibition of enzymes is a challenge for strain engineering and has been achieved so far primarily by random mutation and trial-and-error.
Study Design: A population-based study in Washington State obtained interview data from 969 women aged 45 to 74 years with endometrial cancer diagnosed during 1985 through 1991 or 1994 through 1995 and from 1325 age-matched control subjects selected primarily by random digit dialing.
The evolution of VP3 appears to be driven primarily by random genetic drift coupled with strong negative selection, resulting in low evolution rates.
For highly expressed proteins in eukaryotes, stochasticity arises primarily from random bursts of promoter activation [26], [27], [38], [79] [81] amplified by transcription and translation [28], [32], [34], and turnover of mRNA and protein [38], [82].
Both fixed (primarily) and random effect model methodology was applied.
Previous work (unpublished) and the fact that the DNA sequence tested was primarily not random (i.e. ESTs) led to the multiple, local 7 bp match requirement.
Thus, if we randomly changed synonymous codons in a degenerate codon box, we primarily caused random changes in the nucleotides at the third-codon positions.
Second, the tendency of Aβ42, a natively unfolded polypeptide consist primarily of random-coil structure in their native and soluble states [36], [37], to aggregate may be affected by a combination of genetic [38], environmental [39], and aging factors [40], and the resultant Aβ42 conformers or species may contribute to the heterogeneous pathogenesis of AD [41].
Because of heterogeneity (excess variability) between study results, we used primarily the random-effects model for subsequent sensitivity analyses, which is generally considered to be the more conservative method (Egger et al. 2001).
