Primarily error-free incorporation of dATP was observed for the 5′-T XG-3′ template (template-primer IV); efficient extension to a full-length 18-mer product was also observed when all dNTPs were included in the reaction.
This can cause the optimizer to find parameters that alleviate primarily outlier error contributions, at the expense of overall performance.
By definition, any discrepancy between Tp-PCR and DFM results has been considered primarily an error in Tp-PCR.
The errors for (f=5) also appear to be primarily intensity errors, which will not strongly affect measurements such as peak position estimation.
Most of the inconsistencies happened between neighboring scaffolds, suggesting that errors in the order of Btau_3.1 markers were primarily local errors.
The Dpo4 polymerase exhibited primarily an error-free incorporation of dGTP with the 5′-C XG-3′ template.
Thus, nondisjunction resulting primarily from errors in the first meiotic division in the mother is the most prevalent cause of pregnancy loss and congenital disorders such as Down syndrome in humans (Hassold and Jacobs 1984).
However, despite their advantages, several technical issues – primarily sequencing errors, insufficient coverage of sequences, and potentially anomalous alignment of short-read sequences, have been known to confound the identification of authentic editing sites [ 21, 22].
Although it is difficult to test directly whether tag methylation primarily represents replication errors, such errors probably occur in all cells and should record genealogies in many tissues.
While the lack of alignment introduces possible errors in the inference of the actual evolutionary relationships among species, the lack of assembly primarily introduces sampling error caused by low genome coverage and sequencing errors [ 26, 29].
In addition, primarily consideration of error reporting by systems provides extremely valuable information for prevention of future errors.
