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The FAME obtained was subjected to urea complexation as described previously to remove the saturated fatty acids from the polyunsaturated fatty acids.
These mRNAs were biotinylated with photobiotin and used to subtract the PBMC cDNA library as described previously to remove the housekeeping and non-PBMC-specific genes.
In the subsequent step, SUMO protease I (UlpI, kindly provided by C. D. Lima, Sloan-Kettering Memorial INYtitute, Newasork, NY) was used as discussed previously to remove the SUMO tag and yield untagged peptide.
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CEO of Professional Science Editing for Scientists @ prosciediting.com