For [hu] amyloid, which has been previously found to assemble into a parallel in-register β-sheet structure34, this MPL value corresponds to two PrP23-144 molecules per 0.47-nm β-sheet repeat spacing.
To reflect this choice, we adapted a method that was previously used to assemble Illumina contigs to 454 reads [ 20].
This is particularly relevant in long-term infections, where there is an increasing pressure to activate VSG genes not previously expressed, or to assemble functional VSG gene mosaics.
In addition to assembling the de novo transcriptome of the sepsid fly T. biloba, we used this pipeline to re-assemble previously published transcriptomes that used both 454 and Illumina sequencing platforms.
Atlassian argues that this integration between all of these tools gives development teams "a unified interface to assemble previously siloed information and actions across the entire development workflow — from issues and code-checkins to build status and project progress".
Wu et al. (2010) applied an approach termed 'vdSAR' to assemble previously sequenced small RNA libraries from invertebrates, in order to both demonstrate their sequence overlap, and to classify the infecting viral agents.
From this entire 792 gene set, we used the previously described PAP tool to assemble a core transcriptional regulatory network composed of 6 transcription factor genes (TFs) and 24 target genes, for a total of 55 interactions.
All of the predicted protein-coding genes – together with those of the 62 previously annotated genomes – were used to assemble a database in the program Phamerator (S.G.C., R.W.H, and G.F.H, unpublished), which compares each of the predicted amino acid sequences with all others and sorts the genes into phams [16].
This enables the design and manufacturing of a range of products with unique features that were previously difficult or near impossible to assemble.
The Velvet software package [ 34] was run iteratively following previously established methods [ 26] to assemble reads into contiguous sequences (contigs).
Literature searches were conducted to assemble data on previously established and tested regimens which were given priority.
