Sentence examples for previously substituted from inspiring English sources

We had previously substituted Y110 with the non-phosphorylatable cysteine and found no change in pro-apoptotic function.

To investigate the role of selected amino acids on substrate preference, three amino acids in the GOOX-VN substrate binding site were previously substituted to corresponding residues in chito-oligosaccharide oxidase (ChitO) from Fusarium graminearum[ 15] or carbohydrate oxidase from Microdochium nivale[ 17], which show 45%and42%2% sequence identity to GOOX-VN, respectively [ 7].

One hundred-fold dilutions of serum samples were tested for anti-HEV of the IgG and IgM isotypes as reported previously, substituting species-specific secondary antibodies where necessary.

New adenosine receptor ligands were designed as hybrid structures between previously synthesized substituted dicyanopyridines and aminopyrimidines, yielding two series of cyano-substituted diphenylaminopyrimidines.

We have previously reported substituted 2-aryl-thiazolidine-4-carboxylic acid amides as potent and selective antiproliferative agents for melanoma.

The electrolysis of isatins and cyclic 1,3-diketones in alcohol in an undivided cell results in the formation of the previously unknown substituted 2- 3-hydroxy-2-oxo-2,3-dihydro-1H-indol-3-yl cyclohexane-1,3-diones in 70–85% substance yields and 700–850% current efficiency.

It was previously reported that substituted pyridines exhibited PDE inhibitory activity [41, 42], and 2-amino-pyridin-3-carbonitriles are adenosine receptor ligands [36].

We have previously identified a substituted cyclobutane, Boc5, that acts in vitro and in vivo as a full GLP-1R agonist [26].

As we reported previously, the halogen substituted complexes are highly active against various human cancer cells and nearly exclusively induce apoptotic cell death.

A similar experiment was performed in which FRDA FXN variants N146K, Q148R, I154F, W155R, and R165C (each at 9 μM), which were purified as previously described, were substituted for native FXN.

This behaviour, also previously observed for differently substituted tetracyclic lactams [28], may indicate a different type of interaction of both compounds with the DNA molecule.