In light of the data we present here, suggesting that Vmn1r67 has alleles fixed in M. m. domesticus and M. m. musculus, and the previously reported fixed alleles for Abpa27 in the three subspecies of the house mouse [59], [60], we evaluated linkage disequilibrium (LD) between these two genes.
The previously reported fixed association of sed-sej [ 38] was not found among the 92 Thailand isolates.
As previously reported, chemically fixed insulin secretory granules appeared as hollow spheres with a mean diameter of ∼350 nm.
Dechorionation and antibody hybridization of fixed oocytes were performed as previously reported [20], but for fixed preps with only GFP, ovarioles were separated by rapidly pipetting up and down with a 1000 µl pipette, washed again in PBST, stained in 495 µl PBS plus 5 µl of 10 µg/ml DAPI for 6 minutes, then washed 5 times in PBST prior to mounting in Slowfade Gold.
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The degree of variation between these two NANOG reference sequences within the reading frame is equal to the presumably fixed variation previously reported between NANOG and NANOGP8 in the same region (Booth and Holland 2004; Jeter et al. 2009), calling into question the accuracy of the previous age estimate of 5.2 million years ago for the origin of NANOGP8 (Booth and Holland 2004).
After a 1-h application of PM+Mg2+, the latter solution was washed out and standard Kreb's solution was superfused again for up to 24 h, after which preparations were fixed as previously reported and processed for histology as detailed below.
Importantly, the range of metaphase spindle orientations was consistent with the previously reported cleavage plane orientations of fixed (Kosodo et al., 2004) and live (Konno et al., 2008) mouse APs, which have been observed to be mostly perpendicular to the apical surface.
Aside from the parameter estimates reported below, all other model parameters were considered as system values and fixed at the previously reported values.
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kg is the rate of glycosylation of RBCs, fixed to 8.37 ·10–6 as previously reported.
