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This set of experimental conditions enabled us to accurately determine the number of chromosomes in cells in which new DNA replication rounds are halted and previously replicated chromosomes have finished segregating.
H6N2 and H8N4 strains represented two AIV subtypes isolated from poultry and previously replicated to high titers in LMH cells with visual cell damage indicative of a cytopathic effect (CPE) at 48 h post infection (hpi).
Our study confirmed 5 of 5 previously replicated maternal smoking-related CpG sites representing 3 gene regions.
One fragment of the cytochrome b was previously replicated in the Ancient Biomolecules Centre (Oxford), yielding the same sequence as in the present study.
We observed nominally significant associations between CBC and SNPs in three regions previously associated with breast cancer, 10q26 (FGFR2), 8q24, and 2q35, all of which had been previously replicated in studies of UBC [ 20- 28].
Fourth, any genes lacking polymorphisms (coding and non-coding) between parental strains were excluded from analyses, and lastly, positional overlap with a previously replicated major colitis susceptibility quantitative trait locus (Cdcs1) prioritised Tm3 for targeted analysis.
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We have previously described replicating adenoviruses with Tcf sites in the early promoters [ 11, 12].
As the genes analyzed in the current study were well replicated previously, a two-tailed P value of <0.05 was considered as statistically significant.
At the scalp level, both techniques (autoregressive modeling and wavelet analysis) replicated previously reported increases of iGBRs during the presentation of familiar relative to unfamiliar objects [5], [16], [32].
A few studies utilized samples with early-onset or morbid obesity for discovery, and replicated previously reported association signals on BMI [7], [14] [16], or implicated specific genetic variants such as a recurrent 16p11.2 deletion [17].
Although no novel variants reached genome-wide significance, we replicated previously identified associations with genetic markers near the HNF1B locus.
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