Sentence examples for previously reconstituted from inspiring English sources

These investigations demonstrated that SMA could be used to extract membrane proteins that have been previously reconstituted into bilayers of synthetic phospholipids.

We have previously reconstituted N-WASP and WRC-dependent actin assembly in brain cell extract so that NPF activity generated actin comet tails on PL beads that were consequently propelled through the extract (Koronakis et al., 2011).

Purified protein (5 μg), previously reconstituted with cofactor, was inoculated with substrate (methylmalonyl-CoA, 0.1 mg) and run for several time points as well as overnight at 37 °C and in the dark.

This sequence of events leads to deficient activation, followed by transient proliferation and deletion of donor-reactive T cells, as demonstrated in vivo by injecting systemically allogeneic apoptotic splenocytes into host mice previously reconstituted with allo-reactive TCR transgenic CD4 T cells [ 57].

Previously, we reconstituted the NPC barrier as hydrogels comprising S. cerevisiae FG domains.

Previously, we reconstituted purified mPiezo1 channels into asymmetric DIBs containing DiPhytanoyl-sn-glycero-3-PhosphoCholine (DPhPC) and 1,2-dioleoyl-sn-glycero-3-phosphate (Dobserved obspontaneoustaneous cation-selective channel activity (Coste et al., 2012).

We measured the rate of lysine incorporation using a previously described reconstituted E. coli translation system (Youngman et al., 2004; Gromadski et al., 2006; Zaher and Green, 2009) on a series of lysine-encoding simple mRNAs including: AUG-AAA-UUC-AAG-UAA (MKFK-Stop), AUG-UUC-AAA (MFK), AUG- AAA or AUG- AAAA (MK(A or G)5-Stop).

The J558 cell line has been used previously to reconstitute and study BCR signaling (Dylke et al., 2007; Flaswinkel and Reth, 1994; Jang et al., 2010; Justement et al., 1990).

Chemical analyses were made on the hexane extracts described above, which were previously evaporated and reconstituted in 100 µl of hexane with 10 ng/µl of eicosane (nC20; not present in F. selysi) as an injection internal standard.

We previously established a reconstituted β-secretase assay using the APP 633 685 substrate [ 21].

For example, based on genome-wide microarray analyses, our group previously identified and reconstituted genetic regulatory networks to improve the tolerance of E. coli against isooctane [ 13].