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For Mccp, previously published conventional PCR primers were applied.
We studied the fluid properties of the modified fluid model (DPD Morse) and compared it with a previously published conventional DPD based fluid model.
Chromosome 1q (15/20), chromosome 8 (14/20), chromosome 11 (5/20), chromosome 17q (9/20) and chromosome 20q (6/20) gains (duplications and amplifications), and chromosome 22 (7/20) deletions are the most frequent aberrations, which is consistent with the previously published conventional CGH results [ 2].
The main advantages of this basic ChIP assay over previously published conventional protocols [[ 20, 31, 37], for example] is the combination of speed (only about 2 hours to obtain ChIP samples, via the elimination of some common steps) and sensitivity (known targets could be detected with only about 20,000 cell equivalents were used per PCR reaction).
The presence of BRSV was tested with a modification of a previously published conventional reverse transcriptase polymerase chain reaction (RT-PCR) assay targeting the F-gene with the forward primer sequence 5′-AACCGGCTTCCTTCAGTAGAGC-3′ and the reverse primer sequence 5′-CAATACCACCCACGATCTGTCC-3′[ 15].
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To approach the problem, a simulation model was developed and validated according to the previously published VAPEX conventional and fractured experiments.
Design and process conditions were identified to achieve similar TPCs (0.75 $L−11 for 1000 t d−1 and 0.63 $L−11 for 2000 t d−1, respectively) of sound conceptual designs previously published employing conventional technology.
Previously published series on conventional microscopic TS in patients with acromegaly and a macroadenoma found that the chance of remission after TS could be predicted by the suspected invasiveness of the macroadenoma on the preoperative MRI scan [ 2, 4, 18].
This assumption is supported by a small feasibility study [ 6], where Richetti et al. analysed VMAT in 45 patients with preoperative CRT of LARC and demonstrated improved dose conformality and a tumor downstaging, comparable to previously published data for conventional RT.
Results to date suggest this technique of "hybrid" TAAA repair to be a safe alternative to conventional repair for TAAA in older patients with significant comorbidity, with results in our institution similar to those previously published for younger patients undergoing conventional repair in high-volume centers.
All patients were treated according to two previously published local protocols based on conventional chemotherapy followed by high-dose chemotherapy [ 19- 21].
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