Researchers had previously probed the painting with infrared, neutron and conventional X-ray methods, but could not see the behind the top coat, largely because Rembrandt used the same paint (with the same chemical composition) for the underpainting and the final version.
All the strains have been previously probed for several EAEC-associated genes and verified by HEp-2 adherence assays in our laboratory.
Immunoblotting for β-actin as a loading control was only performed on membranes previously probed for dysbindin-1 and then stripped with the ECL protocol before reprobing for β-actin.
Mg2+ binding has been previously probed with various physicochemical techniques (24, 32, 34, 35, 46).
In order to evaluate MITE distribution within various colonies, we used two probes corresponding to AgaP12MITE205B and AgaP15MITE617, to re-hybridize the Southern blot filter previously probed with the P autonomous subfamilies (Fig. 2b).
Modification of the well-conserved loop separating TM4−5 did not alter Slc11a2 functional expression, providing unambiguous data in a region that had not been previously probed in any Slc11 members.
We have retained a discussion of previous U4/U6 mutagenesis studies, as many of the previously probed residues are directly contacting Prp3 in our crystal structure; however, an influence of the manipulations on Prp3 binding was not considered at that time.
Samples were separated using SDS-PAGE (15% gel), and the proteins transferred to Immobilon-P as described previously, probed with pre-adsorbed Ess1 antibody (Wu et al. 2000) at 1 25,000, and then with True Blot Rabbit HRP (1 2500; Rockland).
The membranes were stripped from the previously hybridized probe and stored in hybridization bags at 4°C to be used with another probe.
The Dfe said its own investigation into the claims was ongoing alongside Birmingham City Council's previously announced probe.
