Advances in engineering of cells and culture formats have led to the development of a new generation of 3D cultures that can recapitulate a variety of multicell-type, morphogenetic behaviors that were previously largely observable only in in vivo settings.
The fluid film stiffening effect, previously only observable by experimentation, could be quantified using the proposed model, as well as the portion of the bearing damping provided by the EHD fluid film.
The embryonic exposures we used for the zebrafish experiments are based on a previously determined observable effect level of p,p′-DDE to enhance domoic acid induced seizures, with the effective dose calibrated to wet-weight body concentration at the time of completed neurodevelopment.
Importantly, estrogenic responses in fish were induced even though concentrations of the individual substances in the mixtures were lower than previously assessed no observable effect concentrations (NOECs) for the individual substances (Thorpe et al. 2001, 2003).
It has previously been observed that inhibiting lysosomal maturation in flies causes a readily observable enlargement of the endosomal compartment [29], in which endocytosed proteins accumulate (e.g., [30]).
Despite severe MPV17 truncation, the homozygous dogs did not manifest any observable phenotypes previously described in other species but were reported to be living normal lives comparable to the heterozygous and wild type dogs.
The ratio of the unique observed to observable peptides is an indicative of the protein abundance, and was previously coined as the protein abundance index-PAI [ 40, 41].
That enabled many more such observations to be made than previously, when such phenomena had been observable only for the few minutes' duration of solar eclipses.
The problem of this technique depth of tissue is thereby minimized and many tumors that were previously hidden are now clearly observable [14].
The aim of this paper is to fill this gap with developing an adaptable, geographical information system-based methodology and implement it in a study area where the previously mentioned conditions are highly observable.
