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The analytical sensitivity of the assay was validated by the recognition of 182 known mutations (153 point mutations, 21 deletions, 7 insertions and 1 duplication) and a cohort of 28 patients were selected to determine the mutation yield, whereby 18 of them were previously negative for mutations in the genes FBN1 and TGFBR2.
Following treatment with oestrogen, P24 staining became positive in 7/13 tumours previously negative for P24, including six tumours which were oestrogen receptor negative.
We detected AChR antibodies to rapsyn-clustered AChR in 66% (25/38) of sera previously negative for binding to AChR in solution and confirmed the results with FACS.
Conversely, it is highly likely that some patients with limbic encephalitis or Morvan's syndrome, previously negative for VGKC antibodies, will prove to have Lgi1 or Caspr2 antibodies.
After an M. bovis outbreak in rhinoceroses and monkeys at a zoo in Louisiana, 7 animal handlers, previously negative for tuberculosis, had positive test results (35 ).
The DMD-CGH allowed us to identify the causative rearrangements in three out of eight DMD patients previously negative for DMD mutations.
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Acquisition was defined as a subject whose nasopharyngeal swab became positive for a bacterium (or serotype) after a previously negative swab for that bacterium (or serotype) [ 15].
In addition, 2 third-follow-up DWI scans, which were previously negative, turned positive for cholesteatoma and was confirmed on surgery.
We determined the infectious agent for 31 (24%) of 131 previously negative samples.
One boy's infection was detected when he was 39 months old, shortly before his death, after previously testing negative for the virus twice.
She had previously tested negative for anti-neutrophil cytoplasmic antibodies (ANCA), rheumatoid factor (RF), anti-DNA and anti-cardiolipin antibodies, hepatitis B and C, HIV and syphilis.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com