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For cloning of the knock-in/knock-out cassette in pBKS− 5ʹ and 3ʹ HR for the knock-in into the ATG of exon, two of Fltp were generated by PCR (453, 454, 455, 456) using the previously mentioned BAC as a template and subcloned into pBKS− using the introduced restriction sites, resulting in pBKS−-Fltp-HomArms.
Listing 2 gives the complete specification of the template of Fig. 3 (the "Button-Text-Image" template), including the logo previously mentioned.
One generic problem intrinsic to the fabrication of patterned nanowires with all of the previously mentioned lithographic methods, is that the nanoscale templates are sacrificial−all the work of producing the pattern is lost in the fabrication of the nanowires, and the templates and/or patterning steps must be repeated to produce each and every duplicate copy of the nanowires.
This does not imply that Krechevsky's and Dodwell's findings are trivial, because other decision strategies are possible that would lead to a different behavioral outcome (i.e., a different stimulus preference), given the same pattern of activation in V1 (e.g., a template-matching strategy, as previously mentioned).
A second round of PCR was performed using the N- and C-terminal fragments as a template with the two outside primers previously mentioned (each at a final concentration of 1 μM).
As previously mentioned, BioHashing [41] without key-binding provides cancelable biometric templates, too.
As previously mentioned, most birds form pairs.
Consider the three examples previously mentioned.
As previously mentioned this was configuration based.
Presumably the musical scores previously mentioned in Vol. 9, Doc.
The Htel de l'Europe is smaller than those previously mentioned.
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