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The endotoxin levels were < 0.1 ng/μg of rhuIL-18 protein that, in our hands, did not previously interfere with in vitro cell-migration experiments [ 37].
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Dominant negative ARF6 has been shown previously to interfere with internalisation of Tfn [15].
Intriguingly, a small molecule previously designed to interfere with Y-box binding protein-1 interactions mediates an anti-inflammatory response and halts fibrogenesis.
It has also been shown previously that NPs interfere with the MTT assay by adsorbing the tetrazolium salt (Wörle-Knirsch et al. 2006), or by releasing metal ions which modify the catalytic activity of the mitochondrial reductases (Kroll et al. 2009).
We show here that of four different herpesviral immunoevasins previously reported to interfere with the MHC class I antigen-presenting pathway, only the HCMV US11 protein strongly downregulates MHC class I expression on the surface of culture-expanded primary hMSCs.
In order to validate the use of TRPV1 activation as a biosensor of AEA cellular uptake, irrespective of the mechanism of this latter process, we compared the effect of several pharmacological tools previously shown to interfere with AEA cellular uptake on AEA -and capsaicin-induced elevation of intracellular Ca2+ as a measure of TRPV1 activity in TRPV1-HEK-293 TRPV1-HEK-293 TRPV1-HEK-293
The instability of the KefFC dimer of dimers in extracts was previously found to interfere with biochemical analysis.
Serum has been previously reported to interfere with transfection efficiency of lipid DNA complexes [ 12, 28, 36].
Compaction and cohesion could be blocked by incubation of MLL-X5C5 cells with the 70 kDa fragment of fibronectin, a fragment previously demonstrated to interfere with FNMA [ 37].
Pharmacological inhibition of HO was previously shown to interfere with the establishment of an SDF-1 gradient between the ischaemic hindlimb and bone marrow, and the mobilization and recruitment of putative EPCs defined as Sca-1+/Kdr+ cells.
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