Exact(12)
Frozen 0.5 g sample of plant tissue (previously ground to a powder in a mortar with liquid N) was homogenized with 5 mL of precooled (-20°C) methanol:water (80 20, v/v) and 2.5 mM Na diethyldithiocarbamate (DDTC).
For GS and GDH analysis, proteins were extracted from 150 mg of fresh matter of leaves (previously ground in a mortar with liquid nitrogen) with 1 mL of extraction buffer (10 mM Na-EDTA, 10 mM MgCl2, 250 mM Tris HCl pH 7.6, 13.3 mM β-mercaptoethanol and 2 mM leupeptine) containing 50 mg of polyvinylpyrrolidone (PVP).
Frozen plant tissue (0.25 g) previously ground using mortar and pestle with liquid nitrogen was homogenized with 20 μL of d4ACC (3 μg/mL in acetonitrile/ acetic acid 0.2% (90/10)) and 3 mL of MeOH/H2O/HCOOH (15/4/1, v/v/v) at -20°C.
The firm, which has three partners and five associates, is now able now to accept large litigation cases involving volumes of documents that "would have previously ground us to a halt," Mr. Kraut said.
The optics are previously ground and polished with continuous polishing process.
The baker's yeast granules were previously ground to a powder form and sieved through a 20-μm sieve.
Similar(48)
Sex served Picasso as an indestructible substitute for the social agreements and systems of belief that had previously grounded art in the world.
If you assume that Morgan's bat was NOT grounded at the time of the bails being broken (which I think is the case - the shoulder of the bat just lifts up again), then the fact that he had previously grounded it is of no importance.
Then NaNO3 (99.0%, Sigma-Aldrich), previously grounded in ball-milling, was added to the isopropanol solution under vigorous stirring.
An amount of 1 g of dried soil, previously grounded and passed through the 100-μm nylon mesh, was heated with 28 mL aqua regia, then filtered through 0.45 μm pore size filter and diluted to 100 mL with ultrapure water.
Kenta Maeda and Enrique Hernandez had previously grounded out to open the inning before Adrian Gonzalez took a swing at the first pitch he saw, resulting in an out to end the inning.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com