Previously established methods have relied upon tissue and cell disruption to measure intracellular H2S levels.
To the knowledge of the authors, no previous experiments have achieved global interlaminar shear strain levels of such magnitudes, and moreover, stress levels were higher than obtained with previously established methods.
The present paper illustrates how the two previously established methods (artificial spring technique and the integro-modal approach) can be combined into a vibroacoustic model in the prediction of the structural acoustic response of complex shaped cavities surrounded by structural boundaries.
We observed that as new methods are introduced, they undergo a rapid method development evolution: within several years the e-resolution score becomes similar for structures obtained from the five methods and they improve from initially poor performance to acceptable quality, comparable with previously established methods, the performance of which is essentially stable.
Toxicity assessment was based on previously established methods [7, 28].
How does the methodology presented here fit in with the previously established methods?
Finally, we present three degenerate kernel methods; the latter is a combination of the two previously established methods in the literature.
Methodologically, the use of SSEM enables the examination of volcanic tephra and the quantification of vesicle properties that were heretofore unavailable to researchers using previously established methods of particle imaging such as standard two-dimensional SEM examination of sectioned and polished lapilli, and X-ray microtomography of clasts or portions of clasts.
The cultivar was then point inoculated at the edge of the plate according to previously established methods (e.g., [39], [64]).
We performed Scyl1 knock down in cultured cells using previously established methods and observed an alteration in Golgi morphology.
Seven days after emergence, Drosophila were tested for resistance to paraquat (Sigma), based on previously established methods [46].
