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Complexes exhibiting the closed form were obtained for both S236A·MgAMPPNP and S236A·MgADP·VO4, where the overall protein topologies are very similar to the previously determined closed forms of S1dC observed in MgADP·VO4 and MgADP·AlF4 complexes (18, 23).
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The closed ATP-AlFx state was previously determined at 4.7 Å resolution (Cong et al, 2010), while the resolutions of the other four states were found to be 10.5 13.9 Å (Supplementary Figure S2).
The new RMSD is close to the previously determined crystal structure and demonstrates the accuracy of TX-MS (Fig. 3b).
These Ea values were close to that previously determined by Verkman for lipid membranes [32].
While RNA degradation rates are structure and length dependent, this value is close to a previously determined half-life of the pT181 antisense RNA (91 nucleotides) of 5 min in vivo.
The packing of TTR molecules in the C2 crystal form and in the previously determined amyloid TTR (ATTR) Leu55Pro crystal structure is close-to-identical.
In addition, the close resemblance of the pseudoduplex structure to previously determined structures of Δ79AAG bound to dsDNA is consistent with the idea that this structure represents a high-affinity complex between AAG and DNA.
After treatment with GCV we found an inhibition for all three viruses with an EC50 of 5.4 8.0 µM, which is close to the EC50 of wild type virus, as previously determined [43].
The average prevalence of S. pneumoniae amongst the infants using cpsA gene-based PCR detection was 78% (95CI: 76%, 83%), very close to 79% (95CI: 75%, 82%) which was previously determined by culture for the same group of infants.
The researchers also set up telescopes at both detector sites and trained them on stars, the closest of which is about 600 light years away, which they had previously determined would send sufficient photons, or starlight, in their direction.
Reaction rate constants with CCl4 and CBr4 are close to the diffusion limit: they are about 4 5 orders of magnitude higher than those previously determined for typical alkyl radicals.
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